Microbial Biotransformations of Bile Acids as Detected by Electrospray Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Microbial biotransformations of bile acids as detected by electrospray mass spectrometry.
Many current experiments investigating the effects of diet, dietary supplements, and pre- and probiotics on the intestinal environments do not take into consideration the potential for using bile salts as markers of environmental change. Intestinal bacteria in vertebrates can metabolize bile acids into a number of different structures, with deamidation, hydroxyl group oxidation, and hydroxyl gr...
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A simple, sensitive, and specific liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) method for the determination of bile acids in human bile has been developed. The bile acids were extracted with a C(18) (octadecyl) reversed-phase column and identified and quantified by simultaneous monitoring of their parent and daughter ions, using the multiple reaction monitoring mode. I...
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We report on the fate of nucleic acids conformation in the gas phase as sampled using native mass spectrometry coupled to ion mobility spectrometry. On the basis of several successful reports for proteins and their complexes, the technique has become popular in structural biology, and the conformation survival becomes more and more taken for granted. Surprisingly, we found that DNA and RNA dupl...
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We present here a tutorial review on the electrospray mass spectrometry technique and its applications to the study of drug-nucleic acid non-covalent complexes. Particular emphasis has been made on the basic principles of the technique, to allow even the non-specialist to design fit-for-purpose mass spectrometry experiments and interpret the results. Standard applications will be described in d...
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Electrospray ionization mass spectrometry (ESI-MS) was applied for the characterization of inorganic polyphosphates [orthophosphate, pyrophosphate, tripolyphosphate, trimetaphosphate, and tetrapolyphosphatel. The high selectivity of ESI-MS allows the detection of different polyphosphate species without preseparation by ion chromatography or capillary electrophoresis. Furthermore, ESI-MS does no...
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ژورنال
عنوان ژورنال: Advances in Nutrition
سال: 2013
ISSN: 2156-5376,2161-8313
DOI: 10.3945/an.112.003061